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Top and middle down MS provided additional information regarding glycosylation sites and different combinational protein species that were not available from oligosaccharide mapping or conventional bottom up analysis. Finally, incorporating a limited enzymatic digestion by immunoglobulin G degrading enzyme of Streptococcus pyogene (IdeS) with MALDI ISD analysis enabled extended sequence coverage of the internal region of protein without pre fractionation. Biological significance: Oligosaccharide profiling together with top and middle down methods enabled: 1) detection of heterogeneous glycosylated protein species and sites in intact IgG1 and fusion proteins with high mass accuracy, 2) estimation of relative abundance levels of protein species in the sample, 3) confirmation of the protein termini structural information, and 4) improved sequence coverage by MALDI ISD analysis for the internal regions of the proteins without sample pre fractionation..

There is fake tan and then there is Amanda Harrington tan. I have stood with the best of them in the Bond position in spray tan salons before a holiday and told myself that the patchy rust colour ‘will do’ and that the fact that my feet look like a five year old has attacked them with an orange paint brush really doesn’t matter. But now Amanda Harrington, the make up artist turned super tanner behind the red carpet glow on Poppy Delevingne, Sienna Miller, Jennifer Aniston et al has developed her own tanning range incorporating her trademark chunky, easy to use face and body brushes which allow you to buff and blend the tan into your skin.

City staff from various departments are working on a review of the draft EIS, including impacts of noise, storm water and other issues. The end result will likely be a response to the EIS from the Mayor’s office. I am also working with affected alders and neighbors.

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