By examining positive and negative ToF SIMS spectra and using PCA, a change in surface chemistry could be detected from 15 seconds of infusion. The intensity of C3H5O2+ peaks in the spectra increased as infusion time increased, indicating that the waxy cuticle of the leaf surface had been removed revealing the underlying epidermal cell layer. Peaks associated with octadecenoic and octadecanoic acids were shown to have a reproducible effect on the positioning of the different infusion times within the PCA plots.

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