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Following exposure to TNF and IL 1, a significant increase in the percentage of wound closure was observed and the increase was higher in IL 1 stimulated cells. Similarly, when tumour cells were exposed to macrophage conditioned media, there was an increase in the percentage of wound closure compared to control cells. The effect of IL 1 and macrophage conditioned media on breast cancer cell migration across blood and lymphatic endothelial cells were also studied using Boyden chamber transmigration assay.

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