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The effects of IL 22 on in vitro MSC proliferation, migration and osteogenic differentiation were evaluated in the presence or absence of IFN and TNF (to ascertain IL 22 activity in pro inflammatory environments). Colorimetric XTT assay, trans well migration assays, quantitative real time polymerase chain reaction (qRTPCR) for MSC lineage markers and osteogenesis assays were used.Results. Combined treatment of MSC with IL 22, IFN and TNF resulted in increased MSC proliferation (p=0.008) and migration (p=0.04); an effect which was not seen in cells treated with IL 22 alone and untreated cells.

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The job market is as competitive as ever. We now a few years into a millennial dominant workforce, and that means certain work practices and expectations are shifting. Companies can no longer compete strictly on the basis of salary and benefits. But in attracting one kind of audience, McCollum argues that these providers are alienating and underserving others without fully realizing it.you just looking at the universe of [traditional] TV watchers, you probably overestimating the importance of sports, McCollum says.Even if other companies wanted to offer sports free packages, their contracts with major broadcast networks might preclude them from doing so. Disney, for instance, might stipulate that a TV provider must carry ABC, Disney channels, and ESPN channels, and require that ESPN be included in the service most popular bundles. A sports free package that didn offer any Disney owned channels could run afoul of contractual obligations if it became too widely adopted.are lots of requirements in contracts about where networks have to be available, and to what percentage of subscribers they have to be available, McCollum says.